We all know that smoking can cause cancer. But did you know that mold also causes cancer and researchers are starting to link the mycotoxins found in moldy tobacco to cancer as well?
If you are a smoker, you might want to know that tobacco is notorious for being infected with mold and mycotoxins.
A pack of cigarettes can look dry, clean, and uniform on the outside while harboring a complex community of fungi and their toxins on the inside. In one classic multi-country survey, researchers isolated “hundreds of strains of fungi” from commercial cigarettes, concluding more than fifty years ago that “cigarettes are contaminated with various fungi.”
The mycotoxins found in mold are not destroyed when tobacco is burned.
They will transfer into the smoke, and the person smoking this mold infected tobacco will ingest these harmful, and potentially deadly toxins into their lungs where it will then enter their blood stream. Even temperature treatments, such as cooking and freezing, do not destroy mold mycotoxins.
More recent work has confirmed that tobacco—whether for cigarettes, cigars, or smokeless products—regularly carries species of Aspergillus, Penicillium, Fusarium, and other molds that can survive curing, storage, and even the burning process.
In controlled storage experiments, cigarettes kept at high humidity for just over a week showed pronounced fungal overgrowth in the tobacco column. Studies of cured and stored tobacco leaf routinely recover Aspergillus niger, Aspergillus flavus, Penicillium species, and Rhizopus, the same genera responsible for visible mildew, off-odors, and mycotoxin contamination in other stored agricultural products.
These findings have significant implications for consumers already concerned about tar, nicotine, and combustion byproducts because mold contamination adds an additional, often overlooked layer of biological and chemical exposure risk.
How Mold Gets Into Tobacco
Mold reaches tobacco at multiple points: in the field, during curing, in storage and manufacturing, and later in a smoker’s home or workplace.
Major contamination pathways include:
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Field and harvest exposure. Tobacco plants provide nutrients and moisture that support fungal colonization, particularly by Aspergillus and Penicillium species, both in the soil and on plant surfaces.
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Curing and fermentation. Air-curing and flue-curing create warm, humid environments where molds can proliferate and alter the leaf’s chemical composition. In moldy cigar tobacco, Aspergillus species have been identified as the dominant mold, representing more than 90 percent of the fungal load in visibly affected leaves.
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Storage and distribution. Cured tobacco leaves and finished products sit in warehouses, factories, and transport containers where even minor moisture excursions can trigger mildew events. Surveys of flue-cured tobacco show a “constant association” between stored leaf and common storage molds like Aspergillus and Penicillium.
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Retail and end-user environments. Research on commercial cigarettes has demonstrated that storage at very high relative humidity for eight days or longer leads to pronounced fungal growth in the tobacco itself. In practice, that risk increases in damp retail back rooms, humidors used incorrectly, and homes with elevated indoor humidity.
Because tobacco is an organic, nutrient-rich matrix, it behaves like other stored agricultural commodities. When temperature and moisture cross key thresholds, molds quickly colonize available surfaces, consume starches and proteins, and generate volatile organic compounds and mycotoxins.
From an environmental health perspective, the tobacco supply chain is a serial opportunity for fungal contamination.
What Happens When Moldy Tobacco Is Smoked
Burning and inhaling a biological material that contains fungi and mycotoxins raises two distinct questions: whether viable organisms or fragments enter the smoke stream, and whether mycotoxins survive combustion or pyrolysis in ways that matter for human health.
Studies examining the microbiology of commercial cigarettes confirm that the tobacco rod contains both bacterial and fungal components under typical storage conditions. When these cigarettes are exposed to high humidity, fungal overgrowth becomes more pronounced, with visible growth and higher counts in culture.
Experimental work on cigarette smoke and microbial toxins indicates that burning contaminated tobacco can generate a mixture of:
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Fungal spores and fragments drawn into mainstream smoke or released into sidestream smoke as particles.
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Microbial cell wall components and toxins that can be carried on particulate matter or in the gas phase.
Smokers as vectors of contaminants
From an indoor environmental standpoint, smokers and smokeless tobacco users can act as vectors, bringing fungal spores, fragments, and mycotoxins into homes, vehicles, and workplaces on:
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Opened packs, loose tobacco, pouches, and cigars.
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Clothing, hands, and surfaces contaminated by handling moldy or dusty tobacco products.
Occupants who do not smoke but share the same environment may therefore be indirectly exposed not only to secondhand smoke but also to trace amounts of mold and mycotoxins associated with tobacco products and their dust.
Indoor Environments, Moldy Tobacco, and Inspection Strategy
For a building science–focused firm, mold in tobacco is relevant because it intersects with indoor air quality, occupant complaints, and source identification.
Rooms and materials impacted by tobacco use
Spaces with heavy or chronic tobacco use often show a predictable pattern:
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Nicotine staining and odor on walls, ceilings, HVAC components, and contents.
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Embedded smoke residue and particulate within carpets, soft furnishings, and dust reservoirs.
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Elevated background particle levels when occupants smoke indoors, especially in smaller or poorly ventilated rooms.
If tobacco itself is mold-contaminated, two additional dynamics can appear:
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Localized mold and odor around storage areas (drawers, cabinets, humidors, closets) where humidity, off-gassing, and tobacco dust combine.
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Increased fungal biomass and mycotoxins in settled dust, especially in areas near storage or frequent use, further complicating efforts to distinguish building-source mold from imported sources.
From an assessment perspective, it becomes important to differentiate between mold arising from building moisture issues and mold introduced via occupant behaviors and products such as tobacco.
Conclusion
Mold in tobacco is not an abstract or rare phenomenon. Decades of research show that cigarettes, cigars, and smokeless tobacco frequently carry Aspergillus, Penicillium, and related storage molds, along with their mycotoxins, including aflatoxins and ochratoxin A.
For environmental and mold professionals, tobacco products represent both a direct exposure medium and a confounding factor when investigating indoor air quality complaints.
References
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Papavassiliou, J., et al. “Cigarette Smoke, Bacteria, Mold, Microbial Toxins, and Chronic Lung Disease.” Chest / PMC article. https://pmc.ncbi.nlm.nih.gov/articles/PMC3136185/
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Zhou, Y., et al. “Analyzing the Quality Differences Between Healthy and Moldy Cigar Tobacco Leaves.” Frontiers in Microbiology (2024). https://pmc.ncbi.nlm.nih.gov/articles/PMC11180791/
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Headspace-SERS assay for early mildewing tobacco leaves. Journal of Raman Spectroscopy (in press). Abstract via ScienceDirect. https://www.sciencedirect.com/science/article/abs/pii/S0039914024010609
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GC-IMS identification of early-warning biomarkers and fungal pathogens causing mold in cigar tobacco leaves. Frontiers in Microbiology (2025). https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1595849/full
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University of Kentucky. “Protect Yourself and Your Workers from Dusts and Molds on Cured Tobacco.” Extension publication. https://burleytobaccoextension.mgcafe.uky.edu/files/tobaccomold_0.pdf
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Soares, C., et al. “Composition and Ecological Functionality of Fungal Communities Associated with Smokeless Tobacco Products.” Microbiology Spectrum (2022). https://journals.asm.org/doi/10.1128/spectrum.02273-21
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Lindholm, J. “Development and Validation of an UPLC–MS/MS Method for Aflatoxins and Ochratoxin A in Tobacco.” CORESTA presentation (2011). https://www.coresta.org/sites/default/files/abstracts/2011_ST15_Lindholm.pdf
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NIOSH. “Adverse Health Effects of Smoking & the Occupational Environment.” U.S. CDC. https://www.cdc.gov/niosh/docs/79-122/default.html
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Zhang, H., et al. “Controlling Mildew of Tobacco Leaf by Bacillus amyloliquefaciens ZH.” Scientific Reports (2025). https://www.nature.com/articles/s41598-025-90058-4
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International Journal of Environmental Research and Public Health. “Determination of Aflatoxin B1 in Smokeless Tobacco Products by UHPLC–MS/MS.” PMC article. https://pmc.ncbi.nlm.nih.gov/articles/PMC5697909/
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U.S. Environmental Protection Agency. “Radioactivity in Tobacco.” https://www.epa.gov/radtown/radioactivity-tobacco
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Larsson, L., et al. “Identification of Bacterial and Fungal Components in Tobacco and Cigarette Smoke.” Environmental Health Perspectives (2008). https://pmc.ncbi.nlm.nih.gov/articles/PMC2556030/
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Molecular and fluorometric based approach for the detection and characterization of fungal and aflatoxin contamination in smokeless tobacco. Toxicology Reports (in press). Abstract via ScienceDirect. https://www.sciencedirect.com/science/article/pii/S0041010125001515
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Evaluations of cigarettes made with mold-damaged and sound flue-cured tobacco. CORESTA / CTTR report. https://reference-global.com/download/article/10.2478/cttr-2013-0363.pdf
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Kwon, O.-S., et al. “Aspergillus-Derived Mycotoxins in Food and the Environment.” Current Opinion in Food Science (2021). https://www.sciencedirect.com/science/article/pii/S2214750021000895

Moe is the CEO and chief mold inspector for Mold Safe Solutions – a Southern California mold inspection and remediation company serving all of San Diego, Riverside and Orange Counties.
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